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PPP1A antibody

Anti-PPP1A antibody used in Western Blot (WB). GTX04684
Anti-PPP1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04684

Cat. No. GTX04684

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse, Rat
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:50-1:200
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.05% proclin300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 230-330 of human PPP1CA (NP_002699.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase 1 catalytic subunit alpha , PP-1A , PP1A , PP1alpha , PPP1A

Cellular Localization

Cytoplasm , Nucleus , nucleoplasm , Nucleolus

Background

The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PPP1A antibody used in Western Blot (WB). GTX04684

GTX04684 WB Image

WB analysis of various samples using GTX04684 PPP1A antibody.
Loading : 25 μg
Dilution : 1:800

Anti-PPP1A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04684

GTX04684 ICC/IF Image

ICC/IF analysis of PC-12 cells using GTX04684 PPP1A antibody.
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 399